Plasmid_Backbone

Part:BBa_K3579016:Design

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF_bb0


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1634
    Illegal SpeI site found at 643
    Illegal SpeI site found at 2442
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 6323
    Illegal SpeI site found at 643
    Illegal SpeI site found at 2442
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 628
    Illegal XhoI site found at 621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1634
    Illegal SpeI site found at 643
    Illegal SpeI site found at 2442
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1634
    Illegal SpeI site found at 643
    Illegal SpeI site found at 2442
    Illegal NgoMIV site found at 6471
    Illegal AgeI site found at 980
    Illegal AgeI site found at 5940
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 3785


Design Notes

BsaI and BbsI restriction sites have been removed



Source

pRMC2 (Plasmid #68940)

References